The research fields of workers of the Department of Biotechnology and Cell Biology are focused on:
- Toxicology in vitro – studying of mechanisms of xenobiotics in biological systems such as tetrabromobisphenol A (TBPA), triclosan (TCS), phthalates, Tris (2, 3-dibromopropyl) isocyanurate (TBC), metallic nanoparticles and others.
- Neurobiology – studying of impact of natural and synthetic substances on cells characterized by neuroectodermal origin such as mouse neurons and astrocytes in vitro.
- Nanotechnology – using metallic nanoparticles as potential therapeutic factors in anticancer therapy, but also studying of methods of targeting delivery of these nanoparticles to cancer cells by liposomal encapsulation.
Scientific and research projects financed from external sources:
Subject: Elastin derivative peptide VGVAPG as a carrier of cytostatic drugs into cancer cells.
Project Manager: Konrad Szychowski Ph.D, DSc. email@example.com
Elastin is one of the main protein responsible for tissue elasticity. Studies have shown that product of its proteolysis is an active peptide characterised by the conserved sequence Val-Gly-Val-Ala-Pro-Gly (VGVAPG). This sequence is easily released from elastin in both physiological and pathological conditions, demonstrating high affinity to the elastin binding protein of the surface receptor, allowing elastin to enter the cell. The aim of the project is to conjugate selected cytostatics with the peptide and assess the impact of the obtained complexes on lungs and breast cancer cells in vitro by investigating their mechanism, including determining the level of expression of selected genes or the level of protein biosynthesis. The obtained results of the project will allow to examine the effectiveness of using elastin-derivative peptide as a drug transporter in targeted anti-cancer therapy in comparison to conventional (unconjugated) cytostatic.
Implementation period: 02.2021 – 07.2021
Financing: Podkarpackie Centrum Innowacji (PCI)
Department of Biotechnology and Cell Biology cooperates with many abroad and national research units, resulting in publishing articles in prestige, international journals, which are characterized by high Impact Factor (IF):
- Yegor Vassetzky, Ph.D., D.Sc., Nuclear Organization and Pathologies CNRS UMR-8126 Institut Gustave Roussy, France;
- Anna Wójtowicz, Ph.D., DSc., Prof.Tit., Department of Animal Nutrition and Biotechnology, and Fisheries, University Of Agriculture in Cracow, Poland;
- Małgorzata Kajta, Ph.D., D.Sc., Prof.Tit., and Agnieszka Wnuk, PhD., Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Science, Cracow, Poland;
- Teresa Leszczyńska, Ph.D., D.Sc., Prof.Tit., and Aneta Kopeć, BEng., PhD., Department of Human Nutrition, University of Agriculture in Cracow, Poland;
- Anna Lewińska, Ph.D., D.Sc. Assoc. Prof., and Maciej Wnuk, Ph.D., D.Sc., Assoc. Prof., Department of Biotechnology, College of Natural Sciences, University of Rzeszow, Poland;
- Agnieszka Ścibior, Ph.D., D.Sc., Assoc. Prof., Laboratory of Oxidative Stress, Catholic University of Lublin, Poland;
- Kamila Rybczyńska-Tkaczyk, BEng., PhD., Department of Environmental Microbiology, University of Life Sciences, Lublin, Poland;
- Tomasz Piechowiak, BEng., PhD., Department of Chemistry and Food Toxicology, Institute of Food Technology and Nutrition, University of Rzeszow, Poland.